Surface translocation of ace2 and tmprss2 upon tlr4/7/8 activation is required for sars-cov-2 infection in circulating monocytes

HIGHLIGHTS

• who: Yi Yao from the All SARS-Co, infection-related work was performed in a Biosafety level , (BSL3) facility at the University of Michigan under the guidance of the Centers for Disease Control and PreventionLNCaP, Vero, and Huh, cell lines were maintained in DMEM supplemented with, % FBS, U/mL penicillin, U/mL streptomycin, and glutamine at , u00b0C with, % CO2. All cell lines tested negative for mycoplasma. SARS-Co, strain WA1/, (BEI resources, Catalog #NR-52281) was added in the , containment facility at a final working dilution equivalent to a multiplicity of infection (MOI) of , and allowed to . . .
  

   

  

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