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- who: Satoru Yabuno from the This study was conducted in accordance with the guidelines of the Institutional Animal Care and Use Committee of Okayama University Faculty of MedicineThe protocol was specifically approved by the Institutional Animal Care and Use Committee of Okayama University Faculty of Medicine (protocol #OKU-202036). All rats were euthanized with an overdose of a mixed solution of, ., mg/kg of medetomidine, ., mg/kg of midazolam, and, ., mg/kg of butorphanol delivered via an intraperitoneal injection at the end of the protocols described below. For MCAO surgery, anesthesia was induced by inhalation of, .0 . . .
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