Syntaxin 7 complexes with mouse vps10p tail interactor 1b, syntaxin 6, vesicle-associated membrane protein (vamp)8, and vamp7 in b16 melanoma cells*

HIGHLIGHTS

• who: Nick Wade from the Cells and Membrane Preparation-, Melanoma cells were kindly provided by DrPeter Parsons (QIMR, Brisbane, Australia) and were cultured in RPMI medium (Life Technologies, Inc.) supplemented with, % fetal calf serum (Life Technologies, Inc.). For immunoblot analysis, total membranes were prepared as follows. Cells were lysed by passage through a, gauge needle in HES buffer (, mM Hepes, ., mM EDTA, mM sucrose) containing protease inhibitors (, ␮g/ml leupeptin, ␮g/ml aprotinin, and , ␮M phenylmethylsulfonyl fluoride) before centrifugation at, ⫻ g for , min. The membrane pellet was resuspended in HES buffer containing, % Triton , and incubated on ice . . .
  

   

  

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