Systematic in vitro specificity profiling reveals nicking defects in natural and engineered crispr-cas9 variants

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  • who: Karthik Murugan and colleagues from the Roy JCarver Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, IA, USA, Molecular, Cellular, and Developmental Biology Interdepartmental Program, Iowa State University, Ames, IA, USA and , Genome Informatics Facility, Office of Biotechnology, Iowa State University, Ames, IA, USA have published the Article: Systematic in vitro specificity profiling reveals nicking defects in natural and engineered CRISPR-Cas9 variants, in the Journal: (JOURNAL)
  • what: The authors employed in vitro cleavage assays of target libraries coupled with highthroughput sequencing to systematically compare cleavage activities and specificities of two . . .

     

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