HIGHLIGHTS
- who: Christophe Gaillochet from the Department of Plant University have published the research work: Systematic optimization of Cas12a base editors in wheat and maize using the ITER platform, in the Journal: (JOURNAL)
- what: To bridge this gap the authors develop ITER (Iterative Testing of Editing Reagents) based on 96-well arrayed protoplast transfections and high-content imaging. The authors show that sequential improvement of five components - NLS crRNA LbCas12a adenine deaminase and linker - leads to a remarkable increase in activity from almost undetectable levels to 40% on an extrachromosomal GFP reporter. The authors demonstrate that . . .
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