Systematic study of single-cell isolation from musculoskeletal tissues for single-sell sequencing

HIGHLIGHTS

  • who: Manman Gao from the Department of Sport Medicine, Inst Translat Med, The First Affiliated Hospital of Shenzhen University, Shenzhen Second People’s Hospital, Shenzhen, China have published the Article: Systematic study of single-cell isolation from musculoskeletal tissues for single-sell sequencing, in the Journal: (JOURNAL)
  • what: The authors designed the rapid cycling enzymatic processing method using tissue-specific enzyme cocktail to treat different human pathological musculoskeletal tissues including degenerated nucleus pulposus (NP) ossifying posterior longitudinal ligament (OPLL) and knee articular cartilage (AC) with osteoarthritis aiming to rapidly and efficiently harvest qualified single-cell . . .

     

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