Target residence of cas9-sgrna influences dna double-strand break repair pathway choices in crispr/cas9 genome editing

HIGHLIGHTS

• who: Si-Cheng Liu from the Institute of Translational Medicine, University People`s Republic of China have published the Article: Target residence of Cas9-sgRNA influences DNA double-strand break repair pathway choices in CRISPR/Cas9 genome editing, in the Journal: (JOURNAL)
• what: The authors demonstrate that weakening target interaction of Cas9-sgRNA promotes the repair bias towards c-NHEJ at the same Cas9-induced DSBs.

SUMMARY

In CRISPR/Cas9 genome editing, targeting Cas9 to a given site is mediated by several interactions, including the contacts between Cas9 and the protospacer . . .

 



If you want to have access to all the content you need to log in!

Thanks :)

Username or E-mail:

If you don't have an account, you can create one here.