Target specificity of the e3 ligase lubac for ubiquitin and nemo relies on different minimal requirements*

HIGHLIGHTS

• who: Judith J. Smit from the EXPERIMENTAL PROCEDURES Construction of Plasmids-Escherichia coli expression constructs of HOIP, HOIPRBR-LDD, and HOIL- L have been described previously (28)Full-length HOIP C A and C A were subcloned from the previously described pcDNA3.1-Myc-HOIP into pGEX- , vectors (GE Healthcare) with an, terminal GST tag for E. coli expression (28). The full-length HOIL- LC A point-mutant was introduced in a HOIL- L pGEX- , construct with the QuikChange Mutagenesis kit from Stratagene (La Jolla, CA). The pASKIBA3plus Strep-NEMO242- , expression construct was kindly provided by Prof . . .
  

   

  

  If you want to have access to all the content you need to log in!

  Thanks :)

  Username or Email

  Password

  Remember me

  Lost your password?

  If you don't have an account, you can create one here.