Targeted demethylation at znf154 promotor upregulates znf154 expression and inhibits the proliferation and migration of esophageal squamous carcinoma cells

HIGHLIGHTS

• who: Wenting He from the Cell culture and, aza-20-deoxycytidine treatment Human esophageal squamous carcinoma cell lines (Kyse, and Kyse-140) were purchased from CellCook Company (Guangzhou, China), and the , T cell line was kindly provided by DrYuan-Zhong Wu (Department of Experimental Research, Sun Yat-sen University). All cell lines were authenticated on the basis of viability, recovery, growth, morphology and isoenzymology by the provider. The ESCC cells were cultured in RPMI , medium (ThermoFisher, Waltham, Massachusetts, USA) and the , T cell line in DMEM (ThermoFisher), each supplemented with, % fetal bovine serum (FBS have published the . . .
  

   

  

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