Targeting double-strand break indel byproducts with secondary guide rnas improves cas9 hdr-mediated genome editing efficiencies

HIGHLIGHTS

• who: Zsolt Bodai from the University of have published the Article: Targeting double-strand break indel byproducts with secondary guide RNAs improves Cas9 HDR-mediated genome editing efficiencies, in the Journal: NATURE COMMUNICATIONS NATURE COMMUNICATIONS
• what: The authors design and test secondary gRNAs targeted to indel sequences with a wide range of frequencies and observe larger improvements in HDR-mediated genome editing efficiencies when targeting higher frequency indel sequences, with no increases in indel rates (in many instances, the authors in fact observe decreases in indel rates). The authors demonstrate the ability of the double . . .
  

   

  

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