Temporally restricted activation of ifnβ signaling underlies response to immune checkpoint therapy in mice

HIGHLIGHTS

• who: Rachael M. Zemek from the Cell culture Cell lines , and , were obtained from CellBank AustraliaCell line Renca was kindly donated by Dr. E. Sotomayor and Dr. F. Cheng (University of South Florida, Tampa, FL). Cell lines were maintained in RPMI , supplemented with , mM HEPES,mM, mercaptoethanol, units/ml penicillin/streptomycin (Thermo Fisher), and, % FCS (Invitrogen, Mulgrave, Australia). Cells were grown to, % before passage and passaged, times before inoculation. Cells were frequently tested for mycoplasma by PCR and remained negative. Cell lines were validated yearly by flow cytometry for MHC class I molecules, Kb (consistent with C . . .
  

   

  

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