The bacterial promoter spacer modulates promoter strength and timing by length, tg-motifs and dna supercoiling sensitivity

HIGHLIGHTS

  • who: Carlo A. Klein from the Universität have published the article: The bacterial promoter spacer modulates promoter strength and timing by length, TG-motifs and DNA supercoiling sensitivity, in the Journal: Scientific Reports Scientific Reports
  • what: This study showed that the GC-content of the spacer seems to play the crucial role in spacer-mediated DNA_supercoiling sensitivity.
  • how: To circumvent this shortcoming the authors developed a mild treatment procedure to investigate long-term promoter activity in living cells. The authors applied core promoter library analysis and transcriptomics to get an insight into . . .

     

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