The ded1 dead box helicase interacts with chk1 and cdc2*

HIGHLIGHTS

• who: Hsing-Yin Liu from the Yeast Strains, Media, and Plasmids-The Spombe strains used in this study are listed in Table I. The cells were grown in YEA medium (15) at , °C except as described. Standard genetic methods were utilized, and most strains were constructed by random spore analysis. The genomic ded, gene was first cloned from a pWH5-based genomic library in a screen of multicopy suppressors of cdc2-r, described elsewhere (5). A, kb HindIII/NsiI fragment was cloned into the p, vector (16) at the HindIII and PstI sites and was shown to . . .
  

   

  

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