The drosophila ftz-f1 nuclear receptor mediates juvenile hormone activation of e75a gene expression through an intracellular pathway

HIGHLIGHTS

• who: Edward B. Dubrovsky from the EXPERIMENTAL PROCEDURES Plasmids, Cell Culture, and Cell Transfection-The pAc51/, His and pMt/V5-His vectors (Invitrogen) were both used for the expression of proteins described in the transfection assays. Coding regions for Manduca sexta JH esterase and Drosophila melanogaster FTZ-F1, MET, and GCE were amplified by PCR using corresponding cDNA clones as templates and inserted into the expression vector. The JHE cDNA was a gift from Dr. B. Hammock (University of California, Davis), and the FTZ-, cDNA was acquired from the Drosophila Genomics Resource Center (expressed sequence tag clone . . .
  

   

  

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