The effect of methanol fixation on single-cell rna sequencing data

HIGHLIGHTS

• who: Xinlei Wang from the (UNIVERSITY) have published the paper: The effect of methanol fixation on single-cell RNA sequencing data, in the Journal: (JOURNAL)
• what: The authors comprehensively evaluated the effect of methanol fixation on single-cell RNAseq results. Although it is often assumed that fixationassociated RNA degradation is the main reason for the discrepancies between live and fixed transcriptomic profiles, the results indicate the incomplete reverse transcription of mRNAs with more complex secondary_structures during the library preparation step may be another important cause of the observed discrepancies. The work determines the feasibility level . . .
  

   

  

  If you want to have access to all the content you need to log in!

  Thanks :)

  Username or E-mail:

  If you don't have an account, you can create one here.