The establishment of transient expression systems and their application for gene function analysis of flavonoid biosynthesis in

HIGHLIGHTS

• who: Bin Xian from the Seeds were collected from Carthamus tinctorius cultivated in the medicinal botanical garden of the Chengdu University of Traditional Chinese Medicine, Chengdu City, Sichuan Province, China, and stored at , u00b0CThe safflower seeds were rinsed under running water for , h, disinfected with, .1% u00adHgCl, for , min, and rinsed three times with sterile water. The sterilized safflower seeds were sown in MS medium (Table, ) under, h light/ dark conditions at , u00b1, u2103 [47]. After , days, sterile safflower seedlings were obtained. Callus was induced from the cotyledons of the sterile safflower seedlings. For safflower callus formation . . .
  

   

  

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