The evolutionary conserved proteins cep90, fopnl, and ofd1 recruit centriolar distal appendage proteins to initiate their assembly

HIGHLIGHTS

SUMMARY

    Using RNA interference (RNAi), the authors showed the involvement of CEP90 in BB anchoring as well as unveiled the interdependent recruitment of CEP90 with OFD1 and FOPNL/FOR20 to build the distal end of the BB in Paramecium. (TIF) S5 Fig Recruitment of CEN2-GFP, CEP90-GFP, OFD1-GFP, and GFP-FOPNL in Cen2, Cen3, and CEP90-depleted cells to newly formed BB. (D) By contrast, Cen3 depletion did not affect neither CEP90-GFP, OFD1-GFP, and GFP-FOPNL recruitment to BB. (TIF) S7 Fig Localization of CEP90, FOPNL, OFD1, CEP83, CEP89, CEP164 in . . .

     

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