The first week following insemination is the period of major pregnancy failure in pasture-grazed dairy cows

HIGHLIGHTS

  • who: D.K. Berg from the ULF samples collected at d, the cellular debris was pelleted by centrifugation at, u00d7 g for , min at, u00b0C, and the supernatant was recoveredThe remaining supernatant was divided into two, mL aliquots. Protease inhibitors (cOmplete, Roche) were added to each aliquot, as recommended by the manufacturer, and then rapidly frozen on dry ice and stored at, u00b0C until analysis. The ULF from cows that did not contain an embryo was analyzed for the presence of IFN-u03c4, which determined the technical flushing error for the , recoveries (i.e., cases where the . . .

     

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