The get pathway serves to activate atg32-mediated mitophagy by er targeting of the ppg1-far complex

HIGHLIGHTS

• who: Mashun Onishi et al. from the expressed endogenously (constructed by Yang Liu, Osaka University)Upon interaction of Atg, with Atg11, SmBiT and LgBiT are brought into close proximity, leading to structural complementation and generation of a luminescent signal. For the assay, cells were grown in glycerol media (SDGCA)., ., units of cells were collected in the early phase of respiration (OD600:, .4-1.6) and washed with , u00b5l PBS. After washing, cells were dissolved in , u00b5l PBS and applied to a, well plate. The detection reagent was prepared by diluting the Nano-Glo Live Cell Substrate (, have . . .
  

   

  

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