The haemophilus ducreyi cytolethal distending toxin induces cell cycle arrest and apoptosis via the dna damage checkpoint pathways*

HIGHLIGHTS

• who: Ximena Cortes-Bratti from the Western Blot Cells were lysed in , ␮l of SDS electrophoresis sample buffer (23), and samples were boiled for , minThe amount of protein in the cell lysates was determined by the Bio-Rad protein assay (Bio-Rad, Hercules, CA). Twenty micrograms of total cell lysate was fractionated by SDS-polyacrylamide gel (23), transferred to polyvinylidene difluoride membranes (Millipore, Bedford, MA) and probed with the respective antibodies. To detect the electrophoretic mobility shift of the chk, kinase, , % linear gradient SDS-polyacrylamide gels were used (Bio-Rad). Blots were developed with enhanced chemiluminescence (ECL . . .
  

   

  

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