The hepatitis c virus non-structural ns5a protein inhibits activating protein⠍1 function by perturbing ras-erk pathway signaling*

HIGHLIGHTS

• who: Andrew Macdonald from the tutes of Health, Bethesda, MD) were amplified by PCR with appropriate sequence-specific primers (sequences available on request) and Pfu polymerase (Promega)PCR-amplified fragments were subcloned into the mammalian expression vector, p, (17). A mutant of NS A in which proline residues, and , were substituted for alanine (PA2) was generated by the PCR overlap method (18) using an appropriate template and overlapping internal oligonucleotide primers. Briefly, internal forward and reverse primers containing the modified sequence were used in conjunction with flanking primers to create two primary PCR products. The primary amplification . . .
  

   

  

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