The heteromultimeric debranching enzyme involved in starch synthesis in arabidopsis requires both isoamylase1 and isoamylase2 subunits for complex stability and activity

HIGHLIGHTS

  • who: Michaela Stettler and colleagues from the Department of Biology, ETH Zurich, Zurich, Switzerland, Institute of Molecular Plant Sciences, University of Edinburgh, Edinburgh, Scotland, United Kingdom, School have published the research work: The Heteromultimeric Debranching Enzyme Involved in Starch Synthesis in Arabidopsis Requires Both Isoamylase1 and Isoamylase2 Subunits for Complex Stability and Activity, in the Journal: PLOS ONE | www.plosone.org of September/30,/2013
  • what: Co-expression of both ISA1 and ISA2 Escherichia coli allowed the formation of the active recombinant enzyme and the authors show using sitedirected mutagenesis that ISA1 is the catalytic . . .

     

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