The human nephrin y1139rsl motif is essential for podocyte foot process organization and slit diaphragm formation during glomerular development

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• who: Eugenel B. Espiritu from the using the QIAquick Gel Extraction Kit (Qiagen, Hilden, Germany)After elution of PCR products from columns, the concentration and purity were measured on the Nanodrop (Thermo Scientific) and sequenced using the same primers as those to amplify the PCR products. Sequencing of purified gelextracted PCR products was only successful on the full-size band in the Control-MO-treated embryos and verified the amplicon expected from the full-size product. Sequencing of the smallest band in the dr-Nephrin-MO PCR products revealed deletion of , bp corresponding to exon, resulting in . . .
  

   

  

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