The human trpa1 intrinsic cold and heat sensitivity involves separate channel structures beyond the n-ard domain

HIGHLIGHTS

• who: Lavanya Moparthi from the Recombinant protein expression and purification The expression and purification of u03941, h, in Pichia pastoris (X-33, Invitrogen) was performed as described previously The gene sequence of h, corresponding to u03941, was optimized for expression in insect cells (GenScript Biotech, Piscataway, NJ, USA). The optimized gene fragment was cloned into the pTriEx, baculovirus donor plasmid. The amplification of the virus and expression of u03941, h, in Hi, and Sf, cells (Invitrogen) were performed at the Protein Production Platform (LP3, Lund University, Sweden). The Hi, cell pellets were lysed using Dounce homogenizer in . . .
  

   

  

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