The impact of hyssop (hyssopus officinalis) extract on activation of endosomal toll like receptors and their downstream signaling pathways

HIGHLIGHTS

• who: Masoumeh Ghasempour from the PBMCs were isolated from heparinized blood samples of , healthy individuals (for all assays) and , patients (for MTT assay only) by Histopaq density gradient centrifugation (Sigma, Missouri, USA) after receiving informed consent from the participantsIn details, the obtained samples were diluted with the same volume of PBS. Then, the Ficoll-Paque Plus (Biosra, France) was carefully added to diluted samples in, :, ratio respectively. The mixture was then centrifuged at , u00d7 g for , min at , u00b0C. The undisturbed PBMC layer was carefully transferred to a new tube and washed twice with three volumes of . . .
  

   

  

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