The lck-14-3-3ζ-trpm8 axis regulates trpm8 function/assembly and promotes pancreatic cancer malignancy

HIGHLIGHTS

• who: Yuan Huang from the Constructs and siRNA The expression constructs for full-length rat Trpm, (NM_134371) in pc, (pcDNA3-TRPM8), pcDNA31-N-Flag (Flag-TRPM8), and pEGFP-, (GFP-TRPM8) were previously described [10, ]. The truncated expression constructs for the GST fused, terminus of , (GST-M C) in pGEX-, GFP fused, terminus of , (GFP-M C) in pEGFP-C1, and Flag-tagged cytosolic domains of , (1, for, N, for, LII, for, LI, and, for, C) subcloned into the pCMV10-3u00d7Flag vector were previously described [47]. The expression constructs for mutant , with mutation Y F was introduced using . . .
  

   

  

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