The neurosteroid pregnenolone promotes degradation of key proteins in the innate immune signaling to suppress inflammation

HIGHLIGHTS

• who: Subathra Murugan from the cells were treated with pregnenolone (, ␮M) or DMSO for , h, followed by lysis of cells in radioimmune precipitation assay buffer and immunoblottingThe membranes were probed with HRP-conjugated anti-FLAG antibody (Sigma) to detect FLAGtagged proteins and HRP-conjugated anti-MYC antibody (Sigma) to detect MYC-CLIP170. The membranes were reprobed with HRP-conjugated anti-actin antibody (Sigma) to detect ␤-actin, which was used as the loading control. Ubiquitination assays HEK T cells (, ⫻ , in, well plates) were co-transfected with, ., ␮g of FLAG-tagged TIRAP or , or MYC-, and , ␮g of HA . . .
  

   

  

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