The nuclear receptor rev-erbα is implicated in the alteration of β-cell autophagy and survival under diabetogenic conditions

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• who: Matthew R. Brown from the INS- E cellsThe rat u03b2-cell line INS- E was provided by Dr. P. Maechler (Department of Cell Physiology and Metabolism, University of Geneva, Geneva, Switzerland) [59]. INS- E cells were grown in , medium with , mM glucose supplemented with, .5% heat-inactivated FBS, mM sodium pyruvate, u03bcM u03b2-mercaptoethanol, mM glutamine, mM HEPES, and , IU/ml penicillin and , u03bcg/ml streptomycin (Life Technologies) at , u00b0C in a humidified, % , atmosphere. No mycoplasma contamination was detected. Soluble and insoluble fractions were prepared using the ReadyPrep Protein Extraction Kit (Soluble/ Insoluble) (Bio-Rad, ) according . . .
  

   

  

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