The simultaneous ex vivo detection of low-frequency antigen-speci w c cd4+ and cd8+ t-cell responses using overlapping peptide pools

HIGHLIGHTS

• who: Cancer Immunol Immunother ( et al. from the Department of Clinical Oncology, Building, P, Leiden University Medical Center, , RC Leiden, The Netherlands have published the Article: The simultaneous ex vivo detection of low-frequency antigen-speci W c CD4+ and CD8+ T-cell responses using overlapping peptide pools, in the Journal: (JOURNAL)
• what: The staining and the analysis were similar as described above for the T-helper ELISPOT.
• how: The data indicated that the presence of other peptides (up to 16 diVerent long peptides)-which theoretically may congest the uptake and processing machinery . . .
  

   

  

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