The structural basis of the ph-homeostasis mediated by the cl−/hco3− exchanger, ae2

HIGHLIGHTS

• who: Qing Zhang from the Expression constructs The different isoforms of human SLC , gene cDNAs were acquired from Han Jiahuai lab at Xiamen University and then cloned into the mammalian cells expression vector pCAGGS (Addgene) modified to introduce a Flag tag plus an , u00d7 His tag followed by a Tobacco Etch Virus (TEV) protease cleavage site at the, terminal of AE The Quickchange site-directed mutagenesis method was used to introduce the mutations. All the mutants and wide-type constructs were confirmed by DNA sequencing before functional and structural studies. have published the paper: The structural basis . . .
  

   

  

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