HIGHLIGHTS
- who: Thilini Abeywansha from the Protein expression and purification The sc, was cloned into a pGEX, p, vector with an, terminal GSTtag and PreScission cleavage site and expressed in BL21(DE3)-RIL cells (Agilent)This RIL strain contains a rare codon plasmid encoding extra copies of argU, ileY, and leuW and overexpresses tRNAArg. Protein production was induced with, ., mM IPTG overnight at , u00b0C in Luria broth (LB). The GST-tagged sc, proteins were purified on glutathione Sepharose B beads (GE Healthcare) in , mM Tris (pH, .5), ., M NaCl, mM MgCl2, and , mM DTT. The GST tag was . . .
If you want to have access to all the content you need to log in!
Thanks :)
If you don't have an account, you can create one here.