The thermal stability and domain interactions of the mannitol permease of escherichia coli

HIGHLIGHTS

• who: Wim Meijberg from the Materials-Dimyristoylphosphatidylcholine (DMPC) was purchased from Avanti Polar LipidsN-Decyl-b-D-maltoside, tosylphenylalanyl chloromethyl ketone-treated trypsin from bovine pancreas and soybean trypsin inhibitor were purchased from Sigma. Nickel-nitrilotriacetic acid-agarose was obtained from Qiagen. Perseitol was purchased from Pfanstiehl Laboratories Inc. (Waukegan, IL). Decyl-PEG was synthesized by B. Kwant at the Department of Chemistry, University of Groningen. Protein Purification-EIImtl and IICmtl were purified as described previously (, ). Construction of the IICBmtl Overexpression System-The plasmid, pMamtlA, containing the wild type mtlA gene and its promoter was used in . . .
  

   

  

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