The thiazide sensitive sodium chloride co-transporter ncc is modulated by site-specific ubiquitylation

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• who: Lena L. Rosenbaek from the AntibodiesRabbit polyclonal antibodies against total , (from Dr. Mark Knepper, NIH, Bethesda, Maryland, USA) or(from Dr. Johannes Loffing, Institute of Anatomy, University of Zurich, Switzerland), phosphorylated Threonine, NCC (pT58)9, FLAG-tag (F7425, Sigma), Actin (A2228, Sigma), Proteasome S (ab3325), and a mouse monoclonal antibody against ubiquitin (P DI, Cell Signaling) were used for immunoblotting. Specificity of p, NCC antibody was confirmed by lack of signal in NCC knockout mouse kidneys (not shown), ablation of immunohistochemcial labeling of the mouse DCT using peptides containing p, but not non-phosphorylated peptides or . . .
  

   

  

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