The translocation assembly module (tam) catalyzes the assembly of bacterial outer membrane proteins in vitro

HIGHLIGHTS

• What: The authors examine the function of the purified E. coli complex in_vitro after reconstituting it into proteoliposomes. The study provides direct evidence that TAM can function as an independent OMP insertase and describes a new method to gain insights into TAM function. All of the experiments shown here were repeated three times with similar results. Based on the in_vitro results, previous genetic, structural and computational studies, and studies on the interactions between the two TAM subunits, the authors propose a model in which a segment of TamB binds to the first POTRA domain of TamA while . . .
  

   

  

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