The trpv4 cation channel mediates stretch-evoked ca2+ influx and atp release in primary urothelial cell cultures

HIGHLIGHTS

• who: Tsutomu Mochizuki from the EXPERIMENTAL PROCEDURES Animals-Wild-type (C BL/ Cr) mice and TRPV4-deficient mice (12) backcrossed on a C BL/ Cr background were usedAll experiments were performed using, to, week-old male mice. All procedures were conducted in accordance with the policies of the Institutional Animal Care and Use Committee, National Institute for Physiological Sciences and University of Yamanashi. Preparation of Primary Urothelial Cell Cultures-Whole bladders were taken from anesthetized mice, and the urothelial cells were prepared by modified procedures (33-35). Briefly, the bladder was inverted by pushing the dome downward . . .
  

   

  

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