The truncated form of flagellin (tflic) provides the 2dcap subunit vaccine with better immunogenicity and protective effects in mice

HIGHLIGHTS

• who: Ying Lu from the CTGu200b GACu200b TCTPorcine kidney epithelial cells (PK-15, ATCC, BNCC, China) were cultured in Dulbecco`s modified Eagle`s medium (DMEM, Thermo, USA) supplemented with, % fetal bovine serum (FBS, Yeasen, China), IU/mL penicillin (Sangon, China) and , mg/L streptomycin (Sangon, China) at , u00b0C in a, % u00ad, atmosphere. The PCV2d JH strain (MG_245867.1) was grown in , cells and utilized for indirect immunofluorescence assay (IFA) and ELISA. Totally , eightweek-old female SPF BALB/c mice and C BL male mice were arbitrarily chosen and purchased from the Experimental Animal Center of Zhengjiang . . .
  

   

  

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