The typically mitochondrial dna-encoded atp6 subunit of the f1f0-atpase is encoded by a nuclear gene in chlamydomonas reinhardtii *

HIGHLIGHTS

  • who: Soledad Funes from the Strain and Culture Conditions-Cell wall-less Creinhardtii strain , was grown in TAP medium (13) with, % sorbitol (Sigma) under continuous light, with agitation at , rpm. The cells were harvested at the late exponential phase of growth. Nucleic Acid Preparation and Sequencing-Total DNA from C. reinhardtii was obtained as previously described (11) or, alternatively, using the DNeasy Plant Mini Kit (Qiagen). Total RNA from C. reinhardtii was obtained using the RNeasy Mini Kit (Qiagen). All standard molecular biology techniques were as described (14). Sequencing was performed by the Kimmel Cancer Center DNA . . .

     

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