The ubiquitin ligase e6-ap is induced and recruited to aggresomes in response to proteasome inhibition and may be involved in the ubiquitination of hsp70-bound misfolded proteins*

HIGHLIGHTS

• who: Amit Mishra from the EXPERIMENTAL PROCEDURES Materials-MG132, (4, dimethylthiazol-2-yl)-2, diphenyltetrazolium bromide (MTT), TRIzol reagent, rabbit polyclonal anti-ubiquitin, anti-luciferase, anti-E6-AP, mouse monoclonal anti-␤-tubulin and anti-luciferase, and all cell culture reagents were obtained from SigmaLipofectamine威, Opti-MEM, ponasterone A, mouse monoclonal , antibody, and the reverse transcription-PCR kit were purchased from Invitrogen. Protein, agarose beads was purchased from Roche Applied Science. Rabbit polyclonal anti-E6-AP, antiGAPDH, mouse monoclonal anti-Hsp70, anti-parkin, antiMyc, and goat polyclonal anti-Hsc, were purchased from Santa Cruz Biotechnology. Rabbit polyclonal anti . . .
  

   

  

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