HIGHLIGHTS
SUMMARY
Zebrafish produce more than 100 eggs per oviposition, and medaka and turquoise killifish, Nothobranchius furzeri, also produce many but fewer eggs than zebrafish (Furutani-Seiki and Wittbrodt, 2004; Skinner and Watt, 2007; Polacik et_al, 2016). The recent emergence of the CRISPR-Cas9 technique (Hwang et_al, 2013; Ablain et_al, 2015; Varshney et_al, 2015) allows the authors to quickly knock out or edit specific genes in various organisms compared with zincfinger nucleases (ZFNs) (Doyon et_al, 2008; Meng et_al, 2008) or transcription activator-like effectors (TALENs) (Huang et_al, 2011; Sander et_al, 2011). This procedure was repeated through . . .
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