The yeast rna-binding protein rbp1p modifies the stability of mitochondrial porin mrna*

HIGHLIGHTS

• who: Leh-Miauh Buu from the Yeast Strains, Media, and Plasmid Construction-The Scerevisiae strain , (MATa ura3, lys2, ade2, trp1-⌬, his3-⌬, leu2-⌬1) was the parental strain used for manipulation and gene disruption. The S. cerevisiae strain , (⫽ YRP582, MATa rpb1, ura, leu2) was a kind gift from Dr. Tien-Hsien Chang (Ohio State University) and was used for mRNA turnover studies. Rich medium contained, % yeast extract, % peptone, and, % glucose or, % galactose. Synthetic minimal medium contained, .7% yeast nitrogen base (without amino acids) and, % glucose or, % galactose and was supplemented with the appropriate nutrients for auxotrophs (23). The . . .
  

   

  

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