The zinc-bed transcription factor bedwarfed promotes proportional dendritic growth and branching through transcriptional and translational regulation in drosophila

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SUMMARY

    To assess the putative role of bdwf in regulating cell type-specific dendritogenesis, the authors used the GAL4-UAS binary expression system for RNAi-mediated knockdown of bdwf in CI, CIII, or CIV md neurons. To dissect the putative effects of Bdwf domains on subcellular localization, the authors generated structure-function mutant transgenes that express either the BED DNA binding domain alone (UAS-bdwf-BED-HA) or a version of bdwf lacking only the BED domain (UAS-bdwf-∆BED-myc). Gal4477, UAS-mCD8::GFP/+ or Gal4477 /UAS-mCD8::GFP/UAS- bdwf -FLAG--HA (L . . .

     

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