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- who: Z Matijasevic from the For the transfection experiments, murine MdmX complementary DNA (cDNA) was placed under the transcriptional control of a conjugate promoter bearing a cytomegaloviral enhancer and chicken-B actin promoter sequences (pCAGGS)Construction of MdmX expression plasmids with MdmX FL and MdmX deletion mutants MdmX-dRING (amino acids, ), and MdmX-dZnF-dRING (amino acids, ) was described previously [52]. Those plasmids were generous gift from Steven J. Berberich (Wright State University). Deletion mutant MdmX-dZnF (d304-323) was generated from the wild-type MdmX cDNA by sitedirected mutagenesis and confirmed by DNA sequencing (GenScript, Piscataway . . .
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