Therapeutic adenine base editing of human hematopoietic stem cells

HIGHLIGHTS

• who: Jiaoyang Liao from the Human CD34+ HSPC transplant and flow cytometry analysis All animal experiments were approved by University Committee on Animal Research Protection of East China Normal UniversityWe had complied with all relevant ethical regulations. CD34+ HSPCs were obtained from anonymized healthy donors or from u03b2hemoglobinopathy patients. NCG-X (NOD-Prkdcem Cd Il2rgem Cd22kitem Cin(V M)/Gpt) mice were ordered from GemPharmatech (Nanjing, China) (Stock, ). Nonirradiated NCG-X female mice (4, weeks of age) were infused by retro-orbital injection with, ., M CD34+ HSPCs (live cells counted immediately before infusion, resuspended in , u03bcl of . . .
  

   

  

  If you want to have access to all the content you need to log in!

  Thanks :)

  Username or Email

  Password

  Remember me

  Lost your password?

  If you don't have an account, you can create one here.