Thermostability enhancement of escherichia coli phytase by error-prone polymerase chain reaction (eppcr) and site-directed mutagenesis

HIGHLIGHTS

• who: March and colleagues from the AKS University, India have published the article: Thermostability enhancement of Escherichia coli phytase by error-prone polymerase chain reaction (epPCR) and site-directed mutagenesis, in the Journal: (JOURNAL)
• what: In this study, we employed epPCR to improve the thermostability of an E. coli phytase.
• how: The authors sought to improve the industrial features of E. coli AppA phytase for feed pelleting thus the authors used a modified epPCR protocol to generate a mutant library followed by directed evolution to enhance thermostability while maintaining high enzyme activity. The . . .
  

   

  

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