Thioredoxin-2 but not thioredoxin-1 is a substrate of thioredoxin peroxidase-1 from drosophila melanogaster

HIGHLIGHTS

• who: Holger Bauer from the Materials-Fruit flies, Dmelanogaster Schneider cell lines and A. gambiae cDNA and cells were kindly provided by Dr. Hans-Michael Müller from the EMBL, Heidelberg, Germany. Escherichia coli Trx and TrxR were gifts from Professor Charles H. Williams, Jr [, ]. University of Michigan. His-tagged human thioredoxin mutant C S (hTrx(C S)) (19) and thioredoxin, from P. falciparum (PfTrx-1) (15) were recombinantly expressed in E. coli. D. melanogaster thioredoxin reductase, (DmTrxR-1) and thioredoxin, (DmTrx-1) were expressed and purified as previously described (3). PCR chemicals and restriction enzymes were . . .
  

   

  

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