Three-dimensional structured illumination microscopy with enhanced axial resolution

HIGHLIGHTS

  • who: Xuesong Li from the (UNIVERSITY) have published the research work: Three-dimensional structured illumination microscopy with enhanced axial resolution, in the Journal: (JOURNAL) of 15/08/2022
  • what: The authors show that placing a mirror directly opposite the sample enables four-beam interference with higher spatial frequency content than 3D SIM illumination, offering near-isotropic imaging with ~120-nm lateral and 160-nm axial resolution. The authors demonstrate the potential of these advances by imaging a variety of cellular samples, delineating the nanoscale distribution of vimentin and microtubule filaments, observing the relative positions of . . .

     

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