HIGHLIGHTS
SUMMARY
Journal of Biomedical Optics December 2022 Other 3D localization approaches have used biplane17 and multiplane18 focusing. Third, because the light field for a single emitter is concentrated in one region in the image plane for a single-spot design, optimized designs have the potential for higher SNR than if the same number of photons are distributed over two spots in the image plane. In the case of the axial localization precision, σ z, the plane at -3 μm is about 1.6× worse than the focal plane, whereas the other edge of the range, at . . .
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