Time-restricted feeding modifies the fecal lipidome and the gut microbiota

HIGHLIGHTS

• who: Bret M. Rust and collaborators from the The University of Minnesota Genome Center (UMGC, Minneapolis, MN, USA) performed extraction and sequencing of S rRNARNA was extracted from mouse fecal pellets (, mg) using a bead-beating method and a MoBio DNA extraction kit. The, regions of S rRNA were sequenced on an Illumina (San Diego, CA, USA) MiSeq stowaway sequencing run. UMGC used a dual indexing sequencing approach of V , regions with KAPA HiFi polymerase that has been previously described [32]. Raw FASTQ data are demultiplexed and trimmed using QIIME , software and then denoised with Dada, software . . .
  

   

  

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