Tips, tricks, and potential pitfalls of crispr genome editing in saccharomyces cerevisiae

HIGHLIGHTS

• who: Saccharomyces cerevisiae et al. from the School of Molecular Biosciences, Washington State University, Pullman, WA, United States, Center for Reproductive Biology have published the research: Tips, Tricks, and Potential Pitfalls of CRISPR Genome Editing in Saccharomyces cerevisiae, in the Journal: (JOURNAL)
• what: While the studies above primarily focused on Cas9 activity in chromatin, a recent report indicates that the activity of other CRISPR enzymes (i.e . The authors discuss how to overcome limitations in Cas9 targeting by using alternative Cas proteins. Interestingly, this approach has also been applied to genome editing experiments in mammalian . . .
  

   

  

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