Tissue- and stage-specific expression of two lipophorin receptor variants with seven and eight ligand-binding repeats in the adult mosquito*

HIGHLIGHTS

• who: Sook-Jae Seo from the were subsequently isolated, and sequencing was performed in the WM. Keck facility (Yale University, New Haven, CT). The deduced amino acid sequence was analyzed using GCG software (University of Wisconsin Genetics Computer Group). Reverse Transcription (RT)- and, ⬘-Rapid Amplification of cDNA Ends (RACE) PCR-Total RNA was extracted from the female fat body and ovary , h PBM using RNeasy mini kits (Qiagen) according to the instructions from the manufacturer. RT-PCR was performed using the Titan One-Step RT-PCR kit (Roche Molecular Biochemicals) with samples of, ., ␮g of total RNA . . .
  

   

  

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